📝 摘要
Tissue microarrays (TMAs) enable high-throughput tissue analysis by selecting a large number of -paraffin-embedded donor tissue block cores and transferring these tissue cores into a positionally encoded array in the recipient TMA block. Once TMAs are constructed, a variety of analysis may be performed on the arrays including histochemical, immunohistochemical, or immunofluorescent staining, and in situ hybridization for DNA or RNA. TMAs offer a cost-effective method for performing parallel analysis of a large number of tissue samples. In this chapter we outline the method of TMA construction with an emphasis on providing useful information in the analysis of a variety of pancreatic neoplasms, including pancreatic adenocarcinomas and pre-invasive lesions. The technique of TMA construction in this chapter is restricted to the use of formalin-fixed paraffin-embedded tissue.
🧾 论文要素
| 作者 | Helen Elaine Remotti |
|---|---|
| 期刊 | Methods in molecular biology |
| 出版机构 | https://openalex.org/P4310319900 |
| 年份 | 2012 |
| 归属类 | 胰腺癌 |
| 平台 | OpenAlex |
| 被引数 | 18 |
| 阅读量 | 11 |
| DOI | 10.1007/978-1-62703-287-2_2 ↗ |
| 关键词 | Tissue microarray; In situ hybridization; Immunohistochemistry; Biology; Computational biology |