Survey of gene amplifications during prostate cancer progression by high-throughout fluorescence in situ hybridization on tissue microarrays.
📄 阅读原文（OpenAlex）↗📝 摘要
Prostate cancer development and progression is driven by the accumulation of genetic changes, the nature of which remains incompletely understood To facilitate high-throughput analysis of molecular events taking place in primary, recurrent, and metastat prostate cancer, we constructed a tissue microarray containing small 0.6-mm cylindrical samples acquired from 371 formalin-fixed blocks, including benign prostatic hyperplasia (n = 32) and primary tumors (n = 223), as well as both locally recurrent tumors (n = 54) and metastases (n = 62) from patients with hormone-refractory disease. Fluorescence in situ hybridization (FISH) was applied to the analysis of consecutive tissue microarray sections with probes for five different genes. High-level (> or =3X) amplifications were very rare (<2%) in primary prostate cancers However, in metastases from patients with hormone-refractory disease, amplification of the androgen receptor gene was seen in 22%, MYC in 11%, and Cyclin-D1 in 5% of the cases. In specimens from locally recurrent tumors, the corresponding percentages were 23, 4, and 8%. ERBB2 and NMYC amplifications were never detected at any stage of prostate cancer progression. In conclusion, FISH to tissue microarray sections enables high-throughput analysis of genetic alterations contributing to cancer development and progression. Our results implicate a role for amplification of androgen receptor in hormonal therapy failure and that of MYC in the metastatic progression of human prostate cancer.
🧾 论文要素
| 作者 | Lukas Bubendorf; Juha Kononen; Pasi A. Koivisto; Peter Schraml; Holger Moch; Thomas Christian Gasser; Niels Willi; Michael J. Mihatsch; G Sauter; Olli Kallioniemi |
|---|---|
| 期刊 | PubMed |
| 出版机构 | https://openalex.org/I1299303238 |
| 年份 | 1999 |
| 归属类 | 前列腺癌 |
| 平台 | OpenAlex |
| 被引数 | 592 |
| 阅读量 | 41 |
| 关键词 | Tissue microarray; Prostate cancer; Prostate; Fluorescence in situ hybridization; Cancer |